Key Features:
- Uses modern browsers, easy to embed and share
- Displays MRI, surfaces, and electrodes in the same canvas
-
Maps multiple subjects on template brains using
AFNI/SUMA(standard 141) orMNI-305locations - Electrode localization
- Volume rendering and surface/electrode animation
- Integration with interactive
R-shinyframework
News | reference page | keyboard shortcuts
System Requirement
-
Web Browsers: the viewer uses
WegGL2to render in browsers. Please check this list to see compatible browsers. As of 2023, Chrome, Firefox, Safari, and Edge (not IE) have full supports.
A. Installation
-
RandRStudio Desktop (Free Version) - Open
RStudio, enter from its console:
install.packages("threeBrain", repos = "https://rave-ieeg.r-universe.dev")If you want to install dev version from Github, then use:
install.packages("remotes")
remotes::install_github("dipterix/threeBrain")- (Optional) Setups: after installation, in
RStudioconsole, type the following command
threeBrain::brain_setup()and follow the instructions.
B. Basic Brain Viewer
Once finishing setting up of threeBrain, there will be a template subject N27 (Collin’s 27) created locally. The location is platform-related. You can find it by running the following command:
library(threeBrain)
default_template_directory()
#> [1] "/Users/dipterix/Library/Application Support/
#> org.R-project.R/R/threeBrain/templates"N27 template folder resides inside of this directory.
Let’s view this subject using the threeBrain function.
- Import subject
library(threeBrain)
n27_path <- file.path(default_template_directory(), "N27")
x <- threeBrain( path = n27_path,
subject_code = 'N27', surface_types = 'pial')- Visualize
plot(x) # alternatively, you can use x$plot()`C. Subject Setup
The sample subject (N27) is a sample generated by FreeSurfer (download). If you have any subjects processed by FreeSurfer, use function threeBrain to visualize.
D. Add/Render Electrodes
If you have electrode file, you can import it before calling plot function. Please make sure it’s in csv format.
x$set_electrodes(electrodes = "[PATH to ELECTRODE FILE]")Here is an example of electrode csv file. Only the first five columns (case-sensitive) are mandatory: Electrode (integer), Coord_x, Coord_y, Coord_z, and Label (character). Coord_* is tkRAS location from FreeSurfer coordinates.
| Electrode| Coord_x| Coord_y| Coord_z|Label | MNI305_x| MNI305_y| MNI305_z|SurfaceElectrode |SurfaceType | Radius| VertexNumber|Hemisphere |
|---------:|-------:|-------:|-------:|:------|--------:|---------:|---------:|:----------------|:-----------|------:|------------:|:----------|
| 1| 29.0| -7.8| -34.6|RMHCH1 | 30.46817| -17.98119| -23.40022|FALSE |pial | 2| -1|left |
| 2| 33.8| -8.0| -34.2|RMHCH2 | 35.57109| -17.76624| -22.80131|FALSE |pial | 2| -1|left |
| 3| 38.0| -7.5| -33.5|RMHCH3 | 39.97102| -16.81249| -22.17986|FALSE |white | 2| -1|right |
| 4| 42.6| -6.8| -33.0|RMHCH4 | 44.79092| -15.73442| -21.82591|FALSE |smoothwm | 2| -1|right |
| 5| 47.0| -6.8| -32.6|RMHCH5 | 49.45370| -15.35431| -21.31272|FALSE |pial | 2| -1|right |
| ...To assign values to electrodes, run
x$set_electrode_values(electrodes = "[PATH to ELECTRODE VALUE FILE]")The electrode value file is also a csv like:
| Electrode| Subject| Project| Time| ValueName| ValueName2| ...|
|---------:|-------:|-------:|-------:|:---------|----------:|-----|
| 1| N27| Demo| 0|A | 30.46817| ...|
| 2| N27| Demo| 0|B | 35.57109| ...|
| 3| N27| Demo| 0|C | 39.97102| ...|
| 4| N27| Demo| 0|D | 44.79092| ...|
| 5| N27| Demo| 0|A | 49.45370| ...|
| ...Project and Time are optional. However, if you are also using rave, please make sure Project exists. If you want to show animation, Time is necessary and must be numeric. ValueName? can be any characters containing letters (A-Z, a-z), letters (0-9) and underscore (_).
E. Merge Subjects and Template mapping
If you have your own subjects with FreeSurfer output, for example, I have two subjects YAB and YCQ. To merge these two subjects and show them on N27 template,
library(threeBrain)
# yab = ... (see section B for import a single subject)
# ycq = ...
template_n27 = merge_brain(yab, ycq, template_subject = 'N27')
plot( template_n27 )The viewer will be in N27 template, and electrodes of these two subjects can be mapped via MNI305 (for surface and stereo EEG) or std.141 (for surface-only).
F. Electrode Localization (YAEL)
YAEL (“Your Advanced Electrode Localizer) has been integrated into RAVE (R Analysis and Visualization of iEEG). Please check our website https://rave.wiki
Citation
To cite threeBrain in publications use:
- Wang, Z., Magnotti, J. F., Zhang, X., & Beauchamp, M. S. (2023). YAEL: Your Advanced Electrode Localizer. Eneuro, 10(10).
- Magnotti, J. F., Wang, Z., & Beauchamp, M. S. (2020). RAVE: Comprehensive open-source software for reproducible analysis and visualization of intracranial EEG data. NeuroImage, 223, 117341.
A BibTeX entry for LaTeX users:
@Article{,
title = {{YAEL}: Your Advanced Electrode Localizer},
author = {Zhengjia Wang and John F. Magnotti and Xiang Zhang and Michael S. Beauchamp},
journal = {Eneuro},
year = {2023},
volume = {10},
number = {10},
publisher = {Society for Neuroscience},
doi = {10.1523/ENEURO.0328-23.2023},
}
@Article{,
title = {{RAVE}: Comprehensive open-source software for reproducible analysis and visualization of intracranial EEG data},
author = {John F. Magnotti and Zhengjia Wang and Michael S. Beauchamp},
journal = {NeuroImage},
year = {2020},
volume = {223},
doi = {10.1016/j.neuroimage.2020.117341},
pages = {117341},
}